LOCUS       Exported                4033 bp ds-DNA    circular SYN 10-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0049max_producer_lasI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Daniel Gerngross
  TITLE     piG0049max
  JOURNAL   Exported 10 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      2..96
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     promoter        150..184
                     /note="const. promoter (J23100)"
     CDS             218..826
                     /codon_start=1
                     /product="LasI (no LVA)"
                     /note="lasI (C0178)"
                     /translation="MIVQIGRREEFDKKLLGEMHKLRAQVFKERKGWDVSVIDEMEIDG
                     YDALSPYYMLIQEDTPEAQVFGCWRIFDTTGPYMLKNTFPELLHGKEAPCSPHIWELSR
                     FAINSGQKGSLGFSDCTLEAMRALARYSLQNDIQTLVTVTTVGVEKMMIRAGLDVSRFG
                     PHLKIGIERAVALRIELNAKTQIALYGGVLVEQRLAVS*"
     terminator      838..924
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      1039..1810
                     /note="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1811..2473
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /note="pBBR1 Rep"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     oriT            2484..2592
                     /note="incP origin of transfer"
     CDS             complement(2738..3928)
                     /note="TetR"
ORIGIN
        1 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
       61 gttctgaggt cattactgga tctatcaaca ggagtccaag actagtcgcc agggttttcc
      121 cagtcacgac gcggccgcaa gcttgcatgt tgacggctag ctcagtccta ggtacagtgc
      181 tagcgcggga agaacccgca acaataagga gggtaatatg atcgttcaga tcggtcgtcg
      241 tgaagagttc gacaaaaaac tgctgggtga aatgcacaaa ctgcgtgctc aggttttcaa
      301 agaacgtaaa ggttgggacg tttccgttat cgacgaaatg gaaatcgacg gttacgacgc
      361 tctgtccccg tactacatgc tgatccagga agacaccccg gaagctcagg ttttcggttg
      421 ctggcgtatc ttcgacacca ccggtccgta catgctgaaa aacaccttcc cggaactgct
      481 gcacggtaaa gaagctccgt gctccccgca catctgggaa ctgtcccgtt tcgctatcaa
      541 ctccggtcag aaaggttccc tgggtttctc cgactgcacc ctggaagcta tgcgtgctct
      601 ggctcgttac tccttgcaga acgacatcca gaccctggtt accgttacca ccgttggtgt
      661 tgaaaaaatg atgatccgtg ctggtctgga cgtttcccgt ttcggtccgc acctgaaaat
      721 cggtatcgaa cgtgctgttg ctctgcgtat cgaactgaac gctaaaaccc agatcgctct
      781 gtacggtggt gttctggttg aacagcgtct ggctgtttcc taataaatta aaggcatcaa
      841 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      901 aacgctctcc tgagtaggac aaatccgccg ccctagacag ctgggcgcgc cccccctacg
      961 ggcttgctct ccgggcttcg ccctgcgcgg tcgctgcgct cccttgccag cccgtggata
     1021 tgtggacgat ggccgcgagc ggccaccggc tggctcgctt cgctcggccc gtggacaacc
     1081 ctgctggaca agctgatgga caggctgcgc ctgcccacga gcttgaccac agggattgcc
     1141 caccggctac ccagccttcg accacatacc caccggctcc aactgcgcgg cctgcggcct
     1201 tgccccatca atttttttaa ttttctctgg ggaaaagcct ccggcctgcg gcctgcgcgc
     1261 ttcgcttgcc ggttggacac caagtggaag gcgggtcaag gctcgcgcag cgaccgcgca
     1321 gcggcttggc cttgacgcgc ctggaacgac ccaagcctat gcgagtgggg gcagtcgaag
     1381 ggcgaagccc gcccgcctgc cccccgagcc tcacggcggc gagtgcgggg gttccaaggg
     1441 ggcagcgcca ccttgggcaa ggccgaaggc cgcgcagtcg atcaacaagc cccggagggg
     1501 ccactttttg ccggaggggg agccgcgccg aaggcgtggg ggaaccccgc aggggtgccc
     1561 ttctttgggc accaaagaac tagatatagg gcgaaatgcg aaagacttaa aaatcaacaa
     1621 cttaaaaaag gggggtacgc aacagctcat tgcggcaccc cccgcaatag ctcattgcgt
     1681 aggttaaaga aaatctgtaa ttgactgcca cttttacgca acgcataatt gttgtcgcgc
     1741 tgccgaaaag ttgcagctga ttgcgcatgg tgccgcaacc gtgcggcacc cctaccgcat
     1801 ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg
     1861 tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa
     1921 acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt
     1981 ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata
     2041 cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg
     2101 caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt
     2161 gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct
     2221 gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc
     2281 gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct
     2341 gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc
     2401 cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac gggtaacgct
     2461 gccgcgccgg tagggccggc ctacggccag cctcgcagag caggattccc gttgagcacc
     2521 gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca
     2581 cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg
     2641 caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc
     2701 ccgaagcagg gttatgcagc ggaaaaggac aattgtctca ggtcgaggtg gcccggctcc
     2761 atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg
     2821 ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag
     2881 tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt
     2941 catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga
     3001 gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc
     3061 ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg
     3121 cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca
     3181 ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg
     3241 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gccacaatgg
     3301 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggac
     3361 ggcgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     3421 tgagcaccgc cgccgcaagg aatggtgcgt gcagggagat ggcgcccaac agtcccccgg
     3481 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     3541 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     3601 cggtgatgcc ggccacgatg cgtccggcgt agagaatcca caggacgggt gtggtcgcca
     3661 tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa
     3721 agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg
     3781 ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc
     3841 ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga
     3901 tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact
     3961 gtgataaact accgcattac agtttatcga tgataagctg tcaaatttaa atcgtaatta
     4021 ttggggaccc ctg
//
